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Analysis of the aconitine alkaloids in traditional Chinese medicines by nonaqueous capillary electrophoresis using a new recording mode
Author(s) -
Zhao Shengguo,
Pan Zhongwei,
Chen Xingguo,
Hu Zhide
Publication year - 2004
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.332
Subject(s) - aconitine , chemistry , chromatography , capillary electrophoresis , aconitum , dissociation constant , alkaloid , stereochemistry , biochemistry , receptor
The determination of three aconitine alkaloids (hypaconitine, aconitine, mesaconitine) in ve traditional Chinese medicines including two Tibetan medicines, Chuanwu, Caowu, Fuzi, Aconitum Tanguticum Maxim and Aconitum Gymnandrum Maxim by non‐aqueous capillary electrophoresis using a new recording mode is described. The dissociation constants of aconitine, mesaconitine and hypaconitine have also been determined by CZE and were 7.71, 6.60 and 6.25, respectively. The separation was achieved by optimizing the applied voltage, the pH and the concentration of the buffer. The electrophoretic medium was 20 m m borax–70% (v/v) methanol (pH 8.5) and an uncoated capillary (50 cm × 75 µm i.d.) was used. Detection was carried out with a UV monitor at 214 nm. The total time for separation and determination was under 13 min. Copyright © 2004 John Wiley & Sons, Ltd.