Premium
Simultaneous determination of caffeic acid and its major pharmacologically active metabolites in rat plasma by LC‐MS/MS and its application in pharmacokinetic study
Author(s) -
Wang Xiangyang,
Li Wei,
Ma Xiaohui,
Chu Yang,
Li Shuming,
Guo Jiahua,
Jia Yumeng,
Zhou Shuiping,
Zhu Yonghong,
Liu Changxiao
Publication year - 2015
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3313
Subject(s) - chemistry , chromatography , formic acid , electrospray ionization , caffeic acid , pharmacokinetics , selected reaction monitoring , analyte , ferulic acid , tandem mass spectrometry , mass spectrometry , protocatechuic acid , antioxidant , pharmacology , biochemistry , medicine
A simple, sensitive and selective high‐performance liquid chromatography electrospray ionization tandem mass spectrometry (LC‐MS/MS) method was developed for simultaneous determination and pharmacokinetic study of caffeic acid (CA) and its active metabolites. The separation with isocratic elution used a mobile phase composed of methanol and water (containing 0.1% formic acid) at a flow rate of 0.2 mL/min. The detection of target compounds was done in selected reaction monitoring (SRM) mode. The SRM detection was operated in the negative electrospray ionization mode using the transitions m/z 179 ([M − H] − ) → 135 for CA, m/z 193 ([M − H] − ) → 134.8 for ferulic acid and isoferulic acid and m/z 153 ([M − H] − ) → 108 for protocatechuic acid. The method was linear for all analytes over the investigated range with all correlation coefficients 0.9931. The lower limits of quantification were 5.0 ng/mL for analytes. The intra‐ and inter‐day precisions (relative standard deviation) were <5.86 and <6.52%, and accuracy (relative error) was between −5.95 and 0.35% ( n = 6). The developed method was applied to study the pharmacokinetics of CA and its major active metabolites in rat plasma after oral and intravenous administration of CA. Copyright © 2014 John Wiley & Sons, Ltd.