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HPLC microdetermination of flurbiprofen enantiomers in plasma with a glycopeptide‐type chiral stationary phase column
Author(s) -
Péhourcq F.,
Matoga M.,
Jarry C.,
Bannwarth B.
Publication year - 2004
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.328
Subject(s) - chemistry , enantiomer , chromatography , calibration curve , high performance liquid chromatography , detection limit , resolution (logic) , phase (matter) , tetrahydrofuran , stereochemistry , organic chemistry , artificial intelligence , computer science , solvent
A rapid and stereospecic HPLC micromethod to quantify urbiprofen enantiomers was developed. Both urbiprofen enantiomers and indomethacin, used as internal standard, were extracted with methylene chloride from 100 µL of acidied plasma. The resolution of the R ‐ and S ‐forms was performed on a bonded vancomycin chiral stationary phase (Chirobiotic V™) with 20% of tetrahydrofuran in ammonium nitrate (100 m m , pH 5) as mobile phase. Calibration curves were linear in the range 0.5–10 µg/mL for both enantiomers. A good accuracy (≤5%) was obtained for all quality controls, with intra‐day and inter‐day variation coefcients equal or less than 7.7%. Recovery of both enantiomers was found in the range 77.4–86.3%. The lower limit of quantitation was 0.25 µg/mL for both enantiomers, without interference of endogenous components. This validated micromethod has been successfully applied for quantifying R ‐urbiprofen and S ‐urbiprofen in rat plasma. Copyright © 2003 John Wiley & Sons, Ltd.