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Target cell extraction coupled with LC‐MS/MS analysis for screening potential bioactive components in Ginkgo biloba extract with preventive effect against diabetic nephropathy
Author(s) -
Qiu Jingying,
Chen Xu,
Zheng Xiaoxiao,
Jiang Xianglan,
Yang Dongzhi,
Yu Yanyan,
Du Qian,
Tang Daoquan,
Yin Xiaoxing
Publication year - 2015
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3264
Subject(s) - chemistry , ginkgo biloba , chromatography , rutin , quercetin , extraction (chemistry) , diabetic nephropathy , tandem mass spectrometry , liquid chromatography–mass spectrometry , pharmacology , mass spectrometry , antioxidant , biochemistry , diabetes mellitus , medicine , endocrinology
A rapid and useful approach for screening potential bioactive components in Ginkgo biloba extract (GBE) with preventive effect against diabetic nephropathy (DN) was developed using mesangial cells extraction coupled with high‐performance liquid chromatography tandem mass spectrometry (LC‐MS/MS) analysis. Mesangial cells were first divided into two groups according to their treatments with high glucose or high glucose plus GBE. After incubation for 4, 8, 12, 16, 24 and 48 h, the cells were harvested and extracted with 40% acetic acid in water before LC‐MS/MS analysis. Then, 19 compounds and five metabolites were found to selectively combine with mesangial cells. Notably, compounds including quercetin and rutin were identified or tentatively characterized according to the results of retention time and MS spectra, which is highly consistent with our previous reports that quercetin and rutin are potent protective agents against glomerulosclerosis in DN. Therefore, all these results indicate that target cell extraction coupled with LC‐MS/MS analysis can be successfully applied for predicting the bioactive components in GBE with preventive effect against DN. Copyright © 2014 John Wiley & Sons, Ltd.