Premium
Liquid chromatographic and mass spectrometric analysis of human serum acid alpha‐1‐glycoprotein
Author(s) -
Kremmer T.,
Szöllösi É.,
Boldizsár M.,
Vincze B.,
Ludányi K.,
Imre T.,
Schlosser G.,
Vékey K.
Publication year - 2004
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.324
Subject(s) - chemistry , chromatography , orosomucoid , oligosaccharide , glycoprotein , mass spectrometry , pngase f , high performance liquid chromatography , biochemistry , glycan
Human serum acid alpha‐1‐glycoprotein (AGP, orosomucoid) content of healthy individuals and cancer patients was measured, isolated and puried using a protocol of fast and biocompatible sample preparation, ion exchange and dye‐ligand afnity chromatographic methods. In comparison to the healthy individuals signicantly higher serum AGP levels were found in a wide spectrum of cancer patients, indicating its diagnostic value in the malignant disease. Oligosaccharide content of AGP samples was separated following PNGase F enzyme digestion and analysed by RP‐HPLC and MALDI‐TOF mass spectrometry. RP‐HPLC and MALDI‐TOF mass spectrometric analysis of sugar constituents of AGP specimen originated from selected cancer patients with high serum AGP levels indicated the appearance of anomal distribution of bi‐, tri‐ and tetra‐antennary oligosaccharide structures compared to the healthy controls. Copyright © 2004 John Wiley & Sons, Ltd.