Premium
Micellar electrokinetic chromatographic determination of rosuvastatin in rabbit plasma and evaluation of its pharmacokinetics and interaction with niacin
Author(s) -
ElKommos Michael E.,
Mohamed Niveen A.,
Ali Hassan R. H.,
Abdel Hakiem Ahmed F.
Publication year - 2014
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3227
Subject(s) - chemistry , chromatography , rosuvastatin , sodium dodecyl sulfate , electrokinetic phenomena , pharmacokinetics , acetonitrile , electrolyte , micellar electrokinetic chromatography , detection limit , pharmacology , electrode , medicine
A specific, accurate, precise and reproducible micellar electrokinetic chromatographic method was developed for in vitro and in vivo estimation of rosuvastatin, a synthetic and potent HMG‐CoA inhibitor, in rabbit plasma. Further, its pharmacokinetics in the presence of niacin, which could be co‐administered for monitoring of severe hypercholestremia, was investigated. The assay procedures involved simple liquid–liquid extraction of rosuvastatin and internal standard, atorvastatin, from a small plasma volume directly into acetonitrile. The organic layer was separated and evaporated under a gentle stream of nitrogen. The residue was reconstituted in the mobile phase and injected electrokinetically into electropherosis system. The background electrolyte consisted of borate buffer (25.0 m m , pH 9.5), 10.0% organic modifier (5.0% methanol + 5.0% acetonitrile) and 25.0 m m sodium dodecyl sulfate at 20.0 kV applied voltage and 215.0 nm detection wavelength for the effective separation of rosuvastatin, niacin and atorvastatin. Copyright © 2014 John Wiley & Sons, Ltd.