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The bioequivalence study of baclofen and lioresal tablets using capillary electrophoresis
Author(s) -
Kowalski Piotr,
Chmielewska Aleksandra,
Konieczna Lucyna,
Olȩdzka Ilona,
Zarzycki Paweł K.,
Lamparczyk Henryk
Publication year - 2004
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.321
Subject(s) - chemistry , chromatography , baclofen , bioequivalence , capillary electrophoresis , detection limit , bioavailability , human plasma , aqueous solution , pharmacology , biochemistry , medicine , receptor , agonist
A simple and robust analytical method for rapid separation and sensitive quantication of baclofen in human plasma by capillary electrophoresis technique was developed. Electrophoretic separation was optimized and successfully performed using simple sodium tetraborate aqueous solution. Observed detection limit in biological material was 10 ng. Using UV detection at 200 nm excellent linearity ( r = 0.999) was observed over the concentration range from 0.025 to 1.0 µg mL −1 . The described method has been validated and applied to the quantitative determination of baclofen in human plasma. The bioavailability of Baclofen (Polpharma) and Lioresal (Novartis) in 18 healthy volunteers was investigated. The results indicate bioequivalence of the reference and Baclofen preparations. Copyright © 2003 John Wiley & Sons, Ltd.