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An HPLC method for the quantitation of 3‐pentylbenzo[c]thiophen‐1(3H)‐one in dog plasma and its application to a pharmacokinetic study
Author(s) -
Li TingTing,
Hua Kai,
Sheng Xiao,
Liu Ling,
Tan JiaNi,
Wang LinNa,
Wei Bo,
Ji Hui,
Zhang YiHua
Publication year - 2014
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3209
Subject(s) - chromatography , chemistry , protein precipitation , beagle , pharmacokinetics , high performance liquid chromatography , calibration curve , plasma , acetonitrile , analytical chemistry (journal) , detection limit , pharmacology , medicine , physics , quantum mechanics
A simple, sensitive and reproducible high‐performance liquid chromatography (HPLC) assay method was developed for the estimation of 3‐pentylbenzo[c]thiophen‐1(3H)‐one (S 5 ), a potential anti‐ischemic stroke agent, in dog plasma. The analytical procedure involves protein precipitation of S 5 and nobiletin (internal standard) from dog plasma with acetonitrile. Chromatographic separation was achieved on Sapphire C18 analytical column with methanol–water (80:20, v/v) as mobile phase. The eluate was monitored using a UV detector set at 260 nm. The calibration curves were linear over the range of 0.2–20 µg/mL. Absolute recoveries of S 5 were 79.2–86.1% from dog plasma. The intra‐ and inter‐day relative standard deviation precisions were <7 and 5%, respectively. The method was successfully applied to the pharmacokinetic study of S 5 in beagle dogs. Copyright © 2014 John Wiley & Sons, Ltd.