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Determination of protein‐unbound rhynchiphylline brain distribution by microdialysis and ultra‐performance liquid chromatography with tandem mass spectrometry
Author(s) -
Lee ChiaJung,
Hsueh Thomas Y.,
Lin LieChwen,
Tsai TungHu
Publication year - 2014
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3206
Subject(s) - chemistry , microdialysis , chromatography , pharmacokinetics , tandem mass spectrometry , high performance liquid chromatography , liquid chromatography–mass spectrometry , mass spectrometry , pharmacology , biochemistry , medicine , extracellular
The stem with hook of Uncaria rhynchophylla (Chinese herbal name Gou‐Teng) is a traditional Chinese medicine that has been ethnopharmacologically used to extinguish wind and clean interior heat. Rhynchophylline (RHY), a tetracyclic oxindole alkaloid isolated from U . rhynchophylla , displays significant antineuroinflammatory effects. However, there is no evidence to indicate that rhynchophylline can cross the blood–brain barrier and be detected in the brain. In this study, an in vivo microdialysis sampling method coupled with UPLC/MS/MS was employed for the continuous simultaneous monitoring of unbound RHY in rat blood and brain. The precursor ion → product ion transition at m/z 385.2 → 160.0 for rhynchophylline was monitored. A calibration curve gave good linearity ( r > 0.996) over the concentration range from 0.5 to 1000 ng/mL. The results demonstrated that rhynchophylline could be detected in the brain and plasma from 15 min to 6 h after its administration (1 or 10 mg/kg, i.v.). All the pharmacokinetic parameters of rhynchophylline in the brain and plasma were obtained. These results show that rhynchophylline can cross the blood–brain barrier and they provide useful clinical information. Copyright © 2014 John Wiley & Sons, Ltd.