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Determination of rhein, baicalin and berberine in traditional Chinese medicinal preparations by capillary electrophoresis with two‐marker technique
Author(s) -
Huitao Liu,
Ketai Wang,
Xingguo Chen,
Zhide Hu
Publication year - 2004
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.317
Subject(s) - baicalin , repeatability , chemistry , berberine , chromatography , capillary electrophoresis , glycyrrhizin , palmatine , coptisine , coptis , high performance liquid chromatography , biochemistry , traditional chinese medicine , pharmacology , medicine , alternative medicine , pathology
Abstract A CZE method for the identication and determination of three bioactive components, rhein, baicalin and berberine, was developed, with 10 m m borate at pH 9.20 as background electrolyte and direct UV detection at 254 nm. The two‐marker (glycyrrhizin acid and cefalexin) technique was used to improve the repeatability of analysis. When the migration indices were used, the repeatability was greatly enhanced compared with migration times. The apparent dissociation constents of rhein and baicalin were also obtained. This method has been successfully applied to the simultaneous analysis of the three components with the recoveries from 96.7% to 104.6%. Copyright © 2004 John Wiley & Sons, Ltd.