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Retracted : Determination of 4‐hydroxy‐2‐nonenal in serum by high‐performance liquid chromatography with fluorescence detection after pre‐column derivatization using 4‐( N , N ‐dimethylaminosulfonyl)‐7‐hydrazino‐2, 1,3‐benzoxadiazole
Author(s) -
Imazato Takahiro,
Shiokawa Akina,
Kurose Yuri,
Katou Yasuha,
Kishikawa Naoya,
Ohyama Kaname,
Ali Marwa Fathy Bakr,
Ueki Yukitaka,
Maehata Eisuke,
Kuroda Naotaka
Publication year - 2014
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3166
Subject(s) - chemistry , chromatography , derivatization , detection limit , extraction (chemistry) , lipid peroxidation , high performance liquid chromatography , rheumatoid arthritis , fluorescence , biochemistry , medicine , antioxidant , physics , quantum mechanics
4‐Hydroxy‐2‐nonenal (4HNE) is a major aldehyde generated during lipid peroxidation. The clinical monitoring of 4HNE in biological fluids should be useful for the early diagnosis of several diseases involving lipid peroxidation, such as rheumatoid arthritis, Parkinson's disease and cancer. In this study, an HPLC with fluorescence detection method was developed for the determination of 4HNE in human serum. The proposed method involves the extraction of 4HNE from human serum by sub‐zero temperature extraction and fluorescent labeling of 4HNE with 4‐( N , N ‐dimethylaminosulfonyl)‐7‐hydrazino‐2, 1,3‐benzoxadiazole. The lower detection limit (signal‐to‐noise ratio = 3) of the method was 0.06 μ m in serum. The proposed method was successfully applied to the measurement of 4HNE in sera obtained from patients with rheumatoid arthritis. Copyright © 2014 John Wiley & Sons, Ltd.