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Metabolite profiling of ginsenoside Rg 1 after oral administration in rat
Author(s) -
Wang Xiaoying,
Wang Chengcheng,
Pu Feifei,
Lin Peiyan,
Qian Tianxiu
Publication year - 2014
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3164
Subject(s) - metabolite , chemistry , ginsenoside , urine , in vivo , ginseng , pharmacology , biotransformation , oral administration , chromatography , biochemistry , enzyme , medicine , biology , alternative medicine , microbiology and biotechnology , pathology
The goal of this study is to investigate the biotransformation of ginsenoside Rg 1 in vivo . A highly sensitive and specific LC‐MS/MS method was developed and used for metabolite identification in rat feces and urine after oral administration of ginsenoside Rg 1 . Four metabolites of Rg 1 were detected in rat feces and three metabolites of Rg 1 were detected in rat urine. Deglycosylation and oxygenation were found to be the major metabolic pathways of ginsenoside Rg 1 after oral administration in rat. Except for the reported metabolites Rh 1 and protopanaxatriol, mono‐oxygenated Rg 1 and mono‐oxygenated protopanaxatriol were detected for the first time after oral administration of Rg 1 . The in vivo metabolite profiling of ginsenoside Rg 1 in rat was proposed. Viewed collectively, Rg 1 was metabolized to mono‐oxygenated Rg 1 , Rh 1 , protopanaxatriol and the secondary metabolite mono‐oxygenated protopanaxatriol in rat. Copyright © 2014 John Wiley & Sons, Ltd.