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Effect of realgar on extracellular amino acid neurotransmitters in hippocampal CA1 region determined by online microdialysis–dansyl chloride derivatization–high‐performance liquid chromatography and fluorescence detection
Author(s) -
Huo Taoguang,
Zhang Yinghua,
Li Weikai,
Yang Huilei,
Jiang Hong,
Sun Guifan
Publication year - 2014
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3154
Subject(s) - realgar , chemistry , microdialysis , high performance liquid chromatography , chromatography , hippocampal formation , derivatization , dansyl chloride , amino acid , extracellular , biochemistry , medicine , mineralogy
An online microdialysis (MD)–dansyl chloride (Dns) derivatization–high‐performance liquid chromatography (HPLC) and fluorescence detection (FD) system was developed for simultaneous determination of eight extracellular amino acid neurotransmitters in hippocampus. The MD probe was implanted in hippocampal CA1 region. Dialysate and Dns were online mixed and derivatized. The derivatives were separated on an ODS column and detected by FD. The developed online system showed good linearity, precision, accuracy and recovery. This online MD‐HPLC system was applied to monitor amino acid neurotransmitters levels in rats exposed to realgar (0.3, 0.9 and 2.7 g/kg body weight). The result shows that glutamate concentrations were significantly increased ( p < 0.05) in hippocampal CA1 region of rats exposed to three doses of realgar. A decrease in γ ‐aminobutyric acid concentrations was found in rats exposed to medium and high doses of realgar ( p < 0.05). Elevation of excitotoxic index (EI) values in hippocampal CA1 region of realgar‐exposed rats was observed ( p < 0.05). Positive correlation was found between EI values and arsenic contents in hippocampus of realgar‐exposed rats, which indicates that the change in extracellular EI values is associated with arsenic accumulation in hippocampus. The developed online MD–Dns derivatization–HPLC–FD system provides a new experimental method for studying the effect of toxic Chinese medicines on amino acid neurotransmitters. Copyright © 2014 John Wiley & Sons, Ltd.