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Quantitative determination of euphol in rat plasma by LC‐MS/MS and its application to a pharmacokinetic study
Author(s) -
Xie Xu,
Li Yongning,
Gao Dongna,
Zhang Yu,
Ren Yanbo
Publication year - 2014
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3151
Subject(s) - chemistry , chromatography , formic acid , pharmacokinetics , selected reaction monitoring , analyte , detection limit , methanol , liquid chromatography–mass spectrometry , bioavailability , mass spectrometry , atmospheric pressure chemical ionization , tandem mass spectrometry , elution , chemical ionization , ionization , ion , pharmacology , medicine , organic chemistry
Euphol is a potential pharmacologically active ingredient isolated from Euphorbia kansui . A simple, rapid, and sensitive method to determine euphol in rat plasma was developed based on liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) for the first time. The analyte and internal standard (IS), oleanic acid, were extracted from plasma with methanol and chromatographied on a C 18 short column eluted with a mobile phase of methanol–water–formic acid (95:5:0.1, v/v/v). Detection was performed by positive ion atmospheric pressure chemical ionization in selective reaction monitoring mode. This method monitored the transitions m / z 409.0 → 109.2 and m / z 439.4 → 203.2 for euphol and IS, respectively. The assay was linear over the concentration range 27–9000 ng/mL, with a limit of quantitation of 27 ng/mL. The accuracy was between –7.04 and 4.11%, and the precision was <10.83%. This LC‐MS/MS method was successfully applied to investigate the pharmacokinetic study of euphol in rats after intravenous (6 mg/kg) and oral (48 mg/kg) administration. Results showed that the absolute bioavailability of euphol was approximately 46.01%. Copyright © 2014 John Wiley & Sons, Ltd.