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Liquid chromatographic enantioseparation of ( RS )‐mexiletine and ( RS )‐fluoxetine using chiral derivatizing reagents synthesized with ( S )‐naproxen moiety
Author(s) -
Batra Sonika,
Bhushan Ravi
Publication year - 2014
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3144
Subject(s) - chemistry , chromatography , moiety , reagent , mexiletine , naproxen , diastereomer , organic chemistry , anesthesia , alternative medicine , pathology , medicine
Enantiomeric separation of racemic mexiletine and fluoxetine was achieved using three chiral derivatizing reagents (CDRs) based on ( S )‐naproxen. Diastereomers were synthesized by reaction of mexiletine or fluoxetine with the CDRs and were separated on a C 18 column under reversed‐phase conditions using a binary mixture of acetonitrile and triethylammonium phosphate/water, with UV detection at 230 and 226 nm. The results obtained for enantioseparation of the two drugs using the three CDRs were compiled and compared. The conditions for derivatization and chromatographic separation were optimized. The method was validated for linearity, repeatability, limit of detection and limit of quantification. Copyright © 2014 John Wiley & Sons, Ltd.