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A method for determination of dimethyl benzoylphenyl urea (BPU) in human plasma by using LC/UV
Author(s) -
Rudek Michelle A.,
Zabelina Yelena,
Zhao Ming,
Wolff Antonio C.,
Baker Sharyn D.
Publication year - 2004
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.314
Subject(s) - chemistry , chromatography , pharmacokinetics , urea , acetonitrile , detection limit , pharmacology , biochemistry , medicine
Dimethyl benzoylphenyl urea (BPU) inhibited tubulin polymerization, caused microtubule depolymerization in vitro and demonstrated activity against solid tumors. BPU is being tested in phase I clinical trials. A rapid and specic method using LC/UV has been developed for quantitation of BPU in human heparin‐containing plasma to perform pharmacokinetic and pharmacodynamic studies. BPU is extracted from plasma into acetonitrile: n ‐butyl‐chloride using paclitaxel as the internal standard and separated on a Waters Symmetry C18 (3.9 × 150 mm, 5 µm) column with acetonitrile–water mobile phase (70:30, v/v) using isocratic ow at 1 mL/min for a run time of 5 min. Ultraviolet detection was utilized and performed at 225 nm for BPU and paclitaxel. The retention times were 1.9 min for paclitaxel and 4.1 min for BPU. Calibration curves were generated over the range of 0.01–10 µg/mL with coefcient of determination of >0.99. The values for within‐day and between‐day precision were ≤17.0% at the LLOQ and ≤7.4% at the low, medium and high quality controls; accuracy was ±5.4%. Following administration of BPU 320 mg as a weekly oral dose to a patient with advanced solid tumor malignancies, the maximum plasma concentration was 2 µg/mL and concentrations were quantiable up to 168 h after administration. The lower limit of quantitation of 0.01 µg/mL allows for successful measurement of plasma concentrations in patients. Copyright © 2004 John Wiley & Sons, Ltd.