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Simultaneous determination of chlorpheniramine and pseudoephedrine in human plasma by liquid chromatography–tandem mass spectrometry
Author(s) -
Chen Xiaoyan,
Zhang Yong,
Zhong Dafang
Publication year - 2004
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.311
Subject(s) - pseudoephedrine , chemistry , chromatography , chlorpheniramine maleate , nasal decongestant , detection limit , analyte , extraction (chemistry) , mass spectrometry , selected reaction monitoring , tandem mass spectrometry , liquid chromatography–mass spectrometry , ephedrine , pharmacology , medicine , anesthesia
A sensitive and specic procedure for simultaneous quantitation of chlorpheniramine and pseudoephedrine in human plasma has been developed and validated. Analytes were extracted from plasma samples by liquid–liquid extraction, separated on a Diamonsil C 18 column (250 × 4.6 mm i.d.) and detected by tandem mass spectrometry with an atmospheric pressure chemical ionization interface. Diphenhydramine was used as the internal standard. The method has a lower limit of quantitation of 0.2 and 2.0 ng/mL for chlorpheniramine and pseudoephedrine, respectively. The intra‐ and inter‐day relative standard deviation, calculated from quality control (QC) samples were below 4.3% for chlorpheniramine and below 9.5% for pseudoephedrine. The inter‐day relative error as determined from QC samples was within 4.7% for each analyte. The overall extraction recoveries of chlorpheniramine and pseudoephedrine were 77 and 61% on average, respectively. The method was successfully applied to pharmaockinetic study of chlorpheniramine and pseudoephedrine in volunteers receiving formulations containing 4 mg of chlorpheniramine maleate and 60 mg of pseudoephedrine hydrochloride. Copyright © 2004 John Wiley & Sons, Ltd.