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One‐step analysis of testis steroidogenesis from neonatal exposure to synthetic estrogen by normal‐phase HPLC
Author(s) -
Kuwada Masahiro,
Kawashima Rei,
Furudate Senich,
Sugano Sachiko,
Maki Jun
Publication year - 2004
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.309
Subject(s) - estrogen , endocrine system , medicine , endocrinology , estrogen receptor , spermatogenesis , juvenile , chemistry , diethylstilbestrol , high performance liquid chromatography , prepuberty , endocrine disruptor , hormone , biology , chromatography , cancer , genetics , breast cancer
Neonatal exposure to synthetic estrogen endocrine disruptors or estrogen‐receptor inhibitors induces developmental abnormalities in the male reproductive system. To investigate whether neonatal exposure affects spermatogenesis in juvenile and pubertal testis, Sprague–Dawley rat pups were given synthetic estrogen endocrine disruptors or estrogen‐receptor inhibitors by a single injection on the day of birth at concentrations ranging between 2 to 40 m m , and sacriced on day 21 (juvenile), 35 (prepuberty) or 50 (puberty). The testes were weighed and examined histologically at each stage. Further, the metabolites of steroidogenesis were analyzed using normal‐phase high performance liquid chromatography. Neonatal exposure signicantly reduced testis weights and steroidogenesis to one‐fth to one‐half of that of the juvenile control, and further suppressed irreversible steroidogenesis and spermatogenesis during puberty. Copyright © 2004 John Wiley & Sons, Ltd.