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HPLC‐fluorescence determination of thiol compounds in the serum of human male and female subjects using HILIC‐mode column
Author(s) -
Kono Yumiko,
Iizuka Hideaki,
Isokawa Muneki,
Tsunoda Makoto,
Ichiba Hideaki,
Sadamoto Kiyomi,
Fukushima Takeshi
Publication year - 2014
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3086
Subject(s) - chemistry , chromatography , thiol , hydrophilic interaction chromatography , fluorescence , column (typography) , high performance liquid chromatography , biochemistry , physics , quantum mechanics , structural engineering , connection (principal bundle) , engineering
High‐performance liquid chromatography–fluorescence detection using a hydrophilic interaction chromatography‐mode column (ZIC®‐HILIC) was used to determine four kinds of thiol compounds in human serum. Sera were obtained from 34 subjects for this study (17 male subjects aged 22–38 years and 17 female subjects aged 18–38 years). Serum cysteine, cysteinylglycine, glutathione, and γ ‐glutamylcysteine, derivatized with ammonium 7‐fluoro‐2,1,3‐benzoxadiazole‐4‐sulfonate, were separated on the ZIC®‐HILIC column and quantified. The serum concentrations of cysteine, cysteinylglycine, glutathione and γ ‐glutamylcysteine were 226 ± 4.7, 23.4 ± 1.3, 3.7 ± 0.2 and 3.2 ± 0.1 μ m , respectively. In addition, the concentrations of serum thiol compounds from male subjects were significantly higher than those of the female subjects ( p < 0.05). Copyright © 2014 John Wiley & Sons, Ltd.