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Pharmacokinetics and oral bioavailability of epimedin C after oral administration of epimedin C and Herba Epimedii extract in rats
Author(s) -
Lee ChiaJung,
Wu YuTse,
Hsueh Thomas Y.,
Lin LieChwen,
Tsai TungHu
Publication year - 2014
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3081
Subject(s) - chemistry , pharmacokinetics , bioavailability , chromatography , oral administration , extraction (chemistry) , analyte , electrospray , mass spectrometry , pharmacology , medicine
Epimedin C, an ingredient of Herba Epimedii, has potential for treatment of cardiovascular disease and bone loss. However, there is still no sensitive analytical method to monitor epimedin C in biological samples. The goal of this study was to develop a sensitive and reliable method based on a LC‐MS/MS for evaluating the pharmacokinetics of epimedin C after administration of Herba Epimedii in rat. Electrospray ionization in positive‐ion mode and multiple reaction monitoring were used to identify and quantitate active components. Analytes were separated by a reverse‐phase C 18 column. Liquid–liquid extraction using ethyl acetate, evaporation and reconstitution was used to plasma sample preparation. Mass transition of precursor ion → product ion pairs were monitored at m / z 823.4 → 313.1 for epimedin C and m / z 237.1 → 178.9 for carbamazepine (internal standard). A calibration curve gave good linearity ( r > 0.999) over the concentration range 2.5–500 ng/mL. Pharmacokinetic data demonstrated that there was rapid distribution and slow elimination after epimedin C administration (1 mg/kg, i.v.). Oral bioavailabilities of epimedin C in the pure compound and in the Herba Epimedii were around 0.58% and 0.13%, respectively. The result suggests that other herbal ingredients of Herba Epimedii may suppress the oral bioavailability of epimedin C. Copyright © 2013 John Wiley & Sons, Ltd.