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Simultaneous determination of three major lignans in rat plasma by LC‐MS/MS and its application to a pharmacokinetic study after oral administration of Diphylleia sinensis extract
Author(s) -
Zhao Chengliang,
Zhang Nan,
He Weiyan,
Li Rui,
Shi Dan,
Pang Li,
Dong Ning,
Xu Hong,
Ji Honglei
Publication year - 2014
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3066
Subject(s) - chemistry , chromatography , electrospray ionization , analyte , triple quadrupole mass spectrometer , mass spectrometry , selected reaction monitoring , calibration curve , tandem mass spectrometry , pharmacokinetics , electrospray , bioanalysis , detection limit , pharmacology , medicine
A sensitive and selective liquid chromatography tandem mass spectrometry was developed and validated for the simultaneous determination of three major lignans (podophyllotoxin, epipodophyllotoxin, and 4′‐demethylpodophyllotoxin) in rat plasma using diphenhydramine as the internal standard. The analytes were detected using a triple quadrupole mass spectrometer that was equipped with an electrospray ionization source in the positive ion and selected reaction monitoring modes. The linearity of the calibration curve was good, with coefficients of determination ( r 2 ) >0.9914 for all of the analytes. The developed method was successfully applied for the simultaneous determination of the three lignans in rat plasma following oral administration of Diphylleia sinensis extract to rats. Copyright © 2013 John Wiley & Sons, Ltd.