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Determination of abamectin and doramectin in sheep faeces using HPLC with fluorescence detection
Author(s) -
Kolar Lucija,
Kužner Jernej,
Eržen Nevenka Kožuh
Publication year - 2004
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.306
Subject(s) - doramectin , abamectin , chromatography , avermectin , detection limit , chemistry , high performance liquid chromatography , feces , repeatability , solid phase extraction , derivatization , ivermectin , pesticide , veterinary medicine , biology , agronomy , microbiology and biotechnology , medicine , anatomy
An analytical procedure for the determination of abamectin and/or doramectin in sheep faeces has been developed. Avermectins were extracted from sheep faeces with acetonitrile, clean‐up using solid phase extraction (SPE) and analysed by high performance liquid chromatography (HPLC) with uorescence detection after derivatization with N ‐methylimidazole. The method has a low detection limit (1.0 ng/g of moist sheep faeces), low quantication limit (2.5 ng/g of moist sheep faeces), good recovery in the range 66.4–80.8% for abamectin and 67.7–85.5% for doramectin as well as good repeatability (>85%). The method is applicable to the study of the time prole of excretion in sheep faeces and also for ecotoxicological studies of both avermectins. Copyright © 2003 John Wiley & Sons, Ltd.