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Development and validation of an LC‐ESI‐MS/MS method for the quantitation of hemslecin A in rhesus monkey plasma and its application in pharmacokinetics
Author(s) -
Bai Min,
Li HongLiang,
He JianChang,
He GongHao,
Feng EnFu,
Liu YueQiong,
Shi PanPan,
Xu GuiLi
Publication year - 2014
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3032
Subject(s) - chemistry , chromatography , electrospray ionization , selected reaction monitoring , pharmacokinetics , ammonium acetate , mass spectrometry , electrospray , extraction (chemistry) , tandem mass spectrometry , liquid chromatography–mass spectrometry , analytical chemistry (journal) , high performance liquid chromatography , medicine
ABSTRACT In this study, a new LC‐ESI‐MS/MS‐based method was validated for the quantitation of hemslecin A in rhesus monkey plasma using otophylloside A as internal standard (IS). Hemslecin A and the IS were extracted from rhesus monkey plasma using liquid–liquid extraction as the sample clean‐up procedure, and were subjected to chromatography on a Phenomenex Luna CN column (150 × 2.0 mm, 3.0 µm) with the mobile phase consisting of methanol and 0.02 mol/mL ammonium acetate (55:45, v/v) at a flow rate of 0.2 mL/min. Detection was performed on an Agilent G6410B tandem mass spectrometer by positive ion electrospray ionization in multiple reaction monitoring mode, monitoring the transitions m/z 580.5 [M + NH 4 ] + → 503.4 and m/z 518.2 [M + NH 4 ] + → 345.0 for hemslecin A and IS, respectively. The assay was linear over the concentration range of 0.5–200 ng/mL and was successfully applied to a pharmacokinetic study in rhesus monkeys. Copyright © 2013 John Wiley & Sons, Ltd.