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The effect of mobile phase composition in the enantioseparation of pharmaceutically relevant compounds with polysaccharide‐based stationary phases
Author(s) -
Sardella Roccaldo,
Ianni Federica,
Lisanti Antonella,
Marinozzi Maura,
Scorzoni Stefania,
Natalini Benedetto
Publication year - 2014
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3015
Subject(s) - chemistry , phase (matter) , selectivity , polymer , alcohol , solvent , enantiomer , resolution (logic) , chromatography , polysaccharide , organic chemistry , catalysis , artificial intelligence , computer science
Mobile phase variables have a deep influence on the chromatographic behavior with polysaccharide‐based chiral stationary phases. Basic additives are generally used to minimize peak broadening arising from unwanted interactions between polar solutes and underivatized silanols. However, basic additives can improve enantioselectivity through disruption of hydrogen bonds and modification of the polymer morphology. Acidic additives are incorporated into the mobile phase during the analysis of acidic compounds as efficiency enhancers. Acidic additives can also improve enantioselectivity by minimizing within the chiral recognition site nonenantioselective retention. Peak shape without acidic additive in the eluent could be severely distorted during the analysis of salified compounds. Concentration and type of alcohol modifier can have an effect on the morphology of the polymer. The different winding of the chiral selector, caused by alcohol modifiers of different size/shape, ultimately results in different stereo environment of the chiral cavities in the polymer chain. Trace amounts of water in normal‐phase eluents can affect retention time, tailing, and resolution. Deliberate addition of water to the eluent can improve peak resolution and save analysis time and solvent needs. Immobilized‐type polysaccharide‐derived chiral stationary phases offer new selectivity profiles and often improved enantioselectivity. Copyright © 2013 John Wiley & Sons, Ltd.