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The capillary electrophoresis separation of benzodiazepine drug using dextran sulfate and SDS as running buffer
Author(s) -
Suzuki Yoshio,
Arakawa Hidetoshi,
Maeda Masako
Publication year - 2004
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.301
Subject(s) - chemistry , chromatography , capillary electrophoresis , electrophoresis , electrokinetic phenomena , dextran , buffer (optical fiber) , boric acid , resolution (logic) , sulfate , analytical chemistry (journal) , telecommunications , organic chemistry , artificial intelligence , computer science
Capillary electrophoresis has been applied the analyses of many clinical drugs due to its rapid, high‐resolution separation. In this study, electrokinetic chromatography involving the combination of SDS and dextran sulfate, which are synthetic polymers, was examined in order to obtain high resolution. Use of 2% dextran sulfate (10,000 molecular weight), 20 m m SDS running buffer containing boric acid solution (pH 9.2) and a silica capillary (inner diameter of 75 µm, effective length of 50 cm, 57 cm overall length) afforded separation of 10 kinds of benzodiazepines. The detection limit was 0.2 µg/mL; additionally, reproducibilities were dened as the peak height and migration time. The average peak height was 5.92% (2.46–17.61), whereas the average migration time was 0.44% (0.18–0.76; n = 5). This separations system can be applied to the analysis and measurement of other pharmaceuticals as well. Copyright © 2004 John Wiley & Sons, Ltd.