Premium
LC‐ESI‐MS/MS analysis and pharmacokinetics of jolkinolide B, a potential antitumor active component isolated from Euphorbia fischeriana , in rat plasma
Author(s) -
Li Yang,
Liu XiaoLi,
Cai ZhenGang,
Zhang SiXi
Publication year - 2014
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3000
Subject(s) - chemistry , chromatography , electrospray ionization , pharmacokinetics , mass spectrometry , electrospray , selected reaction monitoring , active metabolite , tandem mass spectrometry , metabolite , pharmacology , medicine , biochemistry
A simple, specific and reproducible liquid chromatography–electrospray ionization mass spectrometry was developed and validated for the determination of jolkinolide B, a potential antitumor active component isolated from Euphorbia fischeriana , in rat plasma. Chromatographic separation was achieved on a Venusil MP‐C 18 column using an isocratic elution. Jolkinolide B and osthole (internal standard) were monitored by positive electrospray ionization in the selected reaction monitoring mode. Good linearity ( r 2 > 0.996) was achieved by a weighted (1/ x 2 ) linear least‐squares regression over a concentration range of 6.50–2600 ng/mL. The accuracy and precision of the assay were satisfactory and the method proved to be applicable to pharmacokinetics following a single intravenous bolus injection of jolkinolide B to rats. Copyright © 2013 John Wiley & Sons, Ltd.