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Determination of organochlorine compounds in human biological samples by GC‐MS/MS
Author(s) -
Frías Mercedes Moreno,
Torres Margarita Jiménez,
Frenich Antonia Garrido,
Martínez Vidal Jose Luis,
OleaSerrano Fátima,
Olea Nicolás
Publication year - 2004
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.300
Subject(s) - chemistry , chromatography , aldrin , endosulfan , endrin , dieldrin , toxaphene , gas chromatography , elution , detection limit , contamination , environmental chemistry , pesticide , ecology , agronomy , biology
Abstract A study was undertaken to determine the extent of organochlorine pesticide (OP) and polychlorinated biphenyl (PCB) contamination in infant formula milk and in the human milk, fat and serum of women from an agricultural area in Southern Spain. A procedure is proposed that simultaneously detects trace levels of lindane, endosulfan‐ether, vinclozolin, aldrin, endosulfan‐lactone, endosulfan‐alpha, 4,4′DDE, 2,4′DDT, endosulfan‐beta, 4,4′DDT, kepone, endosulfate‐sulfate, methoxychlor, mirex, 2,3,4 PCB, 2,2′,4,5 PCB, 2,3,4,5 PCB and 2,2′,3,3′,6,6′PCB. After liquid–liquid or solid–liquid extraction, the extract of the sample was cleaned by high performance liquid chromatography (HPLC) and the rst eluted fraction was analysed by gas chromatography (GC) with mass spectrometry (MS) detector in tandem mode. To evaluate the validity of the method the following parameters were studied: linearity, detection limits, quantication limits, specicity, percentage recovery and precision. A study of the uncertainty associated with the analytical method was also carried out. Copyright © 2003 John Wiley & Sons, Ltd.