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Screening and determination for potential α ‐glucosidase inhibitory constituents from Dalbergia odorifera T. Chen using ultrafiltration‐LC/ESI‐MS n
Author(s) -
Zhao Chunfang,
Liu Yongqiang,
Cong Dengli,
Zhang Hui,
Yu Jiajia,
Jiang Ying,
Cui Xiuyue,
Sun Jiaming
Publication year - 2013
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.2970
Subject(s) - chemistry , dalbergia , isoliquiritigenin , chromatography , ultrafiltration (renal) , ethyl acetate , liquiritigenin , traditional medicine , biochemistry , medicine , alternative medicine , pathology
In the present study, it was demonstrated that ethyl acetate soluble fraction partitioned from heartwood of Dalbergia odorifera T. Chen (HEF) had a remarkable inhibitory effect on α ‐glucosidase. Therefore HEF was selected as a starting material for screening the potential α ‐glucosidase inhibitors using ultrafiltration liquid chromatography/mass spectrometry (UF‐LC/MS). Twenty‐six compounds were identified with analysis of LC/MS. UF assay indicated that 18 compositions might be α ‐glucosidase inhibitors in HEF; eight of them were estimated for their α ‐glucosidase inhibitory activity, and the results showed that (2 S )‐liquiritigenin, (2 S )‐4′,6‐dihydroxy‐ 7‐methoxyflavanone and isoliquiritigenin displayed obvious inhibition of yeast α ‐glucosidase. In addition, in order to control the quality of HEF, the content of five compounds in HEF was simultaneously determined for the first time. These results provide an important theoretical base for the further application of HEF to treat type 2 diabetes in the clinic and development of natural α ‐glucosidase inhibitors with low toxicity. Copyright © 2013 John Wiley & Sons, Ltd.