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Screening of phage‐displayed human liver cDNA library against doxorubicin with drug‐immobilized monolithic polyacrylamide cryogel
Author(s) -
Yu Xiaoming,
Zhao Peng,
Zhang Lihua,
Zhang Yukui
Publication year - 2013
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.2962
Subject(s) - polyacrylamide , chemistry , phage display , doxorubicin , complementary dna , microbiology and biotechnology , chromatography , biochemistry , combinatorial chemistry , biology , peptide , gene , chemotherapy , polymer chemistry , genetics
ABSTRACT Monolithic polyacrylamide cryogel was prepared and utilized as a new matrix for drug immobilization to screen against phage‐displayed human liver cDNA library. The macropores and hydrophilic nature of the cryogel made it possible for phage particles to pass unhindered. Doxorubicin, an anticancer drug, was covalently bonded to the monolithic cryogel by the glutaraldehyde method, and after five rounds of affinity selection performed in an SPE cartridge, phage clones that displayed Homo sapiens methyl CpG binding protein 2 (MeCP 2 ) were selectively enriched. The interaction between doxorubicin and MeCP 2 displayed phages was further validated by studying the retention of doxorubicin on MeCP 2 phage‐coupled cryogel. These results demonstrate that drug‐coupled polyacrylamide cryogel might be a promising kind of matrix for screening target proteins against phage‐displayed library. Copyright © 2013 John Wiley & Sons, Ltd.