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Validation of a new HPLC‐UV method for determination of the antibiotic linezolid in human plasma and in bronchoalveolar lavage
Author(s) -
Fortuna Serena,
De Pascale Gennaro,
Ragazzoni Enzo,
Antonelli Massimo,
Navarra Pierluigi
Publication year - 2013
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.2947
Subject(s) - chromatography , chemistry , high performance liquid chromatography , bronchoalveolar lavage , extraction (chemistry) , human plasma , chromatographic separation , acetonitrile , accuracy and precision , pharmacokinetics , linear range , analyte , analytical chemistry (journal) , detection limit , pharmacology , medicine , linguistics , philosophy , statistics , mathematics , lung
ABSTRACT A rapid and selective HPLC‐UV method was developed for the quantification of linezolid (LNZ) in human plasma and bronchoalveolar lavage (BAL) at the concentrations associated with therapy. Plasma samples were extracted by solid‐phase extraction followed by evaporation to dryness and reconstitution in mobile phase solution. The chromatographic separation was carried out on a C 18 column with an isocratic mobile phase consisting of dihydrogen phosphate buffer 50 m m (pH 3.5) and acetonitrile (60:40 v/v). The detection was performed using a photodiode array. Under these conditions, a single chromatographic run could be completed within 12 min. The method was validated by estimating the precision and the accuracy for inter‐ and intra‐day analysis in the concentration range of 25–25600 ng/mL. The method was linear over the investigated range with all the correlation coefficients R > 0.999. The intra‐ and inter‐day precision was within 8.90% and the accuracy ranged from −4.76 to +5.20%. This rapid and sensitive method was fully validated and could be applied to pharmacokinetic study for the determination of LNZ levels in human plasma and BAL samples. Copyright © 2013 John Wiley & Sons, Ltd.