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Determination of protoapigenone in rat plasma by high‐performance liquid chromatography with UV detection and its application in pharmacokinetic studies
Author(s) -
Wei Anhua,
Zhou Daonian,
Wu Guanghua
Publication year - 2013
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.2942
Subject(s) - chromatography , chemistry , calibration curve , high performance liquid chromatography , pharmacokinetics , detection limit , methanol , acetic acid , acetonitrile , linear range , plasma , pharmacology , medicine , biochemistry , organic chemistry , physics , quantum mechanics
A simple and sensitive HPLC method using UV detection was developed to determine the concentration of protoapigenone in rat plasma. Chromatographic separation was conducted on a C 18 column with a mobile phase consisting of an acetonitrile–methanol–aqueous phase (containing 0.2% acetic acid, pH 3.0) system at a flow rate of 1.0 mL/min. The UV detector was set at 248 nm. The calibration curve was linear over the range of 0.031–10.0 µg/mL. The lower limit of quantification was 31 ng/mL. The recoveries for plasma samples ranged from 70.3 to 82.5%. The intra‐ and inter‐day accuracy and precision fulfilled the international standards. This method was successfully applied to a pharmacokinetic study of protoapigenone in rats after oral administration of protoapigenone. It was shown that protoapigenone could be absorbed rapidly after oral administration and could reach the maximum concentration within 1 h. Copyright © 2013 John Wiley & Sons, Ltd.