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Development of solid‐phase extraction method and its application for determination of hydrochlorothiazide in human plasma using HPLC
Author(s) -
Zendelovska Dragica,
Stafilov Trajče,
Miloševski Petar
Publication year - 2004
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.293
Subject(s) - hydrochlorothiazide , chromatography , chemistry , extraction (chemistry) , high performance liquid chromatography , detection limit , human plasma , solid phase extraction , phase (matter) , cartridge , materials science , medicine , organic chemistry , blood pressure , metallurgy , radiology
A high‐performance liquid chromatographic method was developed, validated and applied for the determination of hydrochlorothiazide in human plasma. The effects of mobile phase composition, buffer concentration, mobile phase pH and concentration of organic modiers on retention of hydrochlorothiazide and internal standard were investigated. The method involves solid‐phase extraction on RP‐select B cartridges followed by isocratic reversed‐phase chromatography on a Hibar Lichrospher 100 RP‐8 column with UV detection at 230 nm. The recovery, selectivity, linearity, precision and accuracy of the method were evaluated from spiked human plasma samples. Limit of quantication was 10 ng mL −1 . The method has been implemented to monitor hydrochlorothiazide levels in patient samples. Copyright © 2004 John Wiley & Sons, Ltd.