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Determination of leakage from antibody adsorbent: composition analysis and pH effect
Author(s) -
Zhao Lan,
Liu Yongdong,
Wang Yinjue,
Huang Yongdong,
Li Xiunan,
Li Yan,
Ma Guanghui,
Su Zhiguo
Publication year - 2013
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.2929
Subject(s) - chemistry , leakage (economics) , adsorption , chromatography , matrix (chemical analysis) , ligand (biochemistry) , kinetics , sepharose , complex matrix , organic chemistry , enzyme , biochemistry , receptor , quantum mechanics , economics , macroeconomics , physics
To study the leakage at different solution pH values, IgG Sepharose 6FF®, a commercially available immunoadsorbent, was used as a model. The leaked substance consists of three parts: (1) ligands and its fragments; (2) ligands plus matrix fragments in which ligands are chemically attached to the adsorbent matrix; and (3) matrix fragments. Buffer solution pH values had a great effect on both the kinetics and the amount of ligand leakage. Cross‐linking of the adsorbent matrix could reduce both matrix leakage and antibody leakage at pH 3.0, but its effect was limited at pH 11.0 for ligand leakage. Copyright © 2013 John Wiley & Sons, Ltd.