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LC‐MS/MS determination of bakkenolide D in rats plasma and its application in pharmacokinetic studies
Author(s) -
Dai Dongmei,
Pei Lixia,
Tang Lin,
Chen Feng,
Chen Xiuping
Publication year - 2013
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.2927
Subject(s) - chemistry , pharmacokinetics , chromatography , bioavailability , selected reaction monitoring , liquid chromatography–mass spectrometry , oral administration , detection limit , tandem mass spectrometry , mass spectrometry , pharmacology , medicine
A sensitive and rapid liquid chromatography–tandem mass spectrometry (LC‐MS/MS) method was developed and validated for determination of bakkenolide D (BD), which was further applied to assess the pharmacokinetics of BD. In the LC‐MS/MS method, the multiple reaction monitoring mode was used and columbianadin was chosen as internal standard. The method was validated over the range of 1–800 ng/mL with a determination coefficient >0.999. The lower limit of quantification was 1 ng/mL in plasma. The intra‐ and inter‐day accuracies for BD were 91–113 and 100–104%, respectively, and the inter‐day precision was <15%. After a single oral dose of 10 mg/kg of BD, the mean peak plasma concentration of BD was 10.1 ± 9.8 ng/mL at 2 h. The area under the plasma concentration–time curve (AUC 0–24 h ) was 72.1 ± 8.59 h ng/mL, and the elimination half‐life ( T 1/2 ) was 11.8 ± 1.9 h. In case of intravenous administration of BD at a dosage of 1 mg/kg, the AUC 0–24 h was 281 ± 98.4 h⋅ng/mL, and the T 1/2 was 8.79 ± 0.63 h. Based on these results, the oral bioavailability of BD in rats at 10 mg/kg is 2.57%. Copyright © 2013 John Wiley & Sons, Ltd.