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Normal and polar‐organic‐phase high‐performance liquid chromatographic enantioresolution of omeprazole, rabeprazole, lansoprazole and pantoprazole using monochloro‐methylated cellulose‐based chiral stationary phase and determination of dexrabeprazole
Author(s) -
Dixit Shuchi,
Dubey Rituraj,
Bhushan Ravi
Publication year - 2014
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.2915
Subject(s) - chemistry , chromatography , lansoprazole , pantoprazole , rabeprazole , omeprazole , cellulose , enantiomer , phase (matter) , elution , organic chemistry , proton pump inhibitor , medicine , biochemistry
Enantioresolution of four anti‐ulcer drugs (chiral sulfoxides), namely, omeprazole, rabeprazole, lansoprazole and pantoprazole, was carried out by high‐performance liquid chromatography using a polysaccharide‐based chiral stationary phase consisting of monochloromethylated cellulose (Lux cellulose‐2) under normal and polar‐organic‐phase conditions with ultraviolet detection at 285 nm. The method was validated for linearity, accuracy, precision, robustness and limit of detection. The optimized enantioresolution method was compared for both the elution modes. The optimized method was further utilized to check the enantiomeric purity of dexrabeprazole. Copyright © 2013 John Wiley & Sons, Ltd.