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Simultaneous determination of sulfamethoxazole and trimethoprim in human plasma by capillary zone electrophoresis
Author(s) -
Teshima Daisuke,
Otsubo Kaori,
Makino Kazutaka,
Itoh Yoshinori,
Oishi Ryozo
Publication year - 2004
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.291
Subject(s) - trimethoprim , sulfamethoxazole , chemistry , chromatography , capillary electrophoresis , pneumocystis carinii , detection limit , pneumonia , antibiotics , biochemistry , medicine , pneumocystis jirovecii
A capillary electrophoretic method for the simultaneous determination of sulfamethoxazole and trimethoprim in plasma was developed. Sulfamethoxazole and trimethoprim extracted from human plasma with ethyl acetate were analyzed at 20 kV and 25°C using 15 m m phosphate buffer (pH 6.2) as the electrolyte. The detection was by UV at 220 nm. The run time was 8.0 min and the limit of quantication was 10.00 µg/mL for sulfamethoxazole and 2.00 µg/mL for trimethoprim. The recovery was >99% for both compounds. This method enabled the detection of sulfamethoxazole and trimethoprim in plasma of patients after oral ingestion of their combined formulation. The present simple and rapid method is applicable to drug monitoring in immunocompromised patients who are taking the combined formulation of these compounds for the treatment or prophylaxis of Pneumocystis carinii pneumonia. Copyright © 2004 John Wiley & Sons, Ltd.