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Simultaneous quantification of paeoniflorin, nobiletin, tangeretin, liquiritigenin, isoliquiritigenin, liquiritin and formononetin from Si‐Ni‐San extract in rat plasma and tissues by liquid chromatography–tandem mass spectrometry
Author(s) -
Li Tianxue,
Yan Zhixiang,
Zhou Chen,
Sun Jian,
Jiang Chuan,
Yang Xinghao
Publication year - 2013
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.2904
Subject(s) - liquiritigenin , liquiritin , formononetin , chemistry , paeoniflorin , chromatography , isoliquiritigenin , nobiletin , analyte , mass spectrometry , liquid chromatography–mass spectrometry , electrospray ionization , tandem mass spectrometry , selected reaction monitoring , high performance liquid chromatography , daidzein , alternative medicine , flavonoid , pathology , genistein , medicine , antioxidant , biochemistry
ABSTRACT In this study, a sensitive and reliable liquid chromatography–tandem mass spectrometry (LC‐MS/MS) method was developed and validated for the determination of seven bioactive components including paeoniflorin, nobiletin, tangeretin, liquiritigenin, isoliquiritigenin, liquiritin and formononetin in rat plasma and tissues after oral administration of Si‐Ni‐San extract using astragaloside IV as internal standard (IS). The plasma and tissue samples were extracted by solid‐phase extraction. Chromatographic separation was accomplished on a C 18 column with a multiple‐step gradient elution. The quantification was obtained by scanning with multiple reaction monitoring via an electrospray ionization source that was operated by switching between the positive and negative modes in two MS/MS scan segments. Full validation of the assay was implemented. In conclusion, this method demonstrated good linearity and specificity. The lower limits of quantification for the analytes were <7.5 ng/mL. Intra‐ and inter‐day precisions (RSD) were <12.5% and accuracy (RE) ranged from −10.2 to 7.3%. The average recoveries of the analytes from rat plasma and tissues were >65.2% and 58.6%, respectively. The validated method was further applied to the determination of actual rat plasma and tissues after oral administration of Si‐Ni‐San extract. The results provided a meaningful basis for the clinical application of this prescription. Copyright © 2013 John Wiley & Sons, Ltd.