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High‐performance liquid chromatographic determination of Levetiracetam in human plasma: comparison of different sample clean‐up procedures
Author(s) -
Pucci Vincenzo,
Bugamelli Francesca,
Mandrioli Roberto,
Ferranti Anna,
Kenndler Ernst,
Raggi Maria Augusta
Publication year - 2004
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.289
Subject(s) - levetiracetam , chromatography , chemistry , extraction (chemistry) , sample preparation , solid phase extraction , clean up , human plasma , high performance liquid chromatography , methanol , yield (engineering) , materials science , organic chemistry , neuroscience , metallurgy , epilepsy , biology
Abstract An accurate and precise high‐performance liquid chromatographic method using diode array detection for the determination of the novel antiepileptic, Levetiracetam, has been developed. Three clean‐up procedures for the analysis of Levetiracetam in human plasma were implemented and evaluated, namely solid‐phase extraction, deproteinization by addition of organic solvents and formation of insoluble salts. Adenosine was used as the internal standard for all three sample pretreatment procedures. Among the several cartridges used for solid‐phase extraction, the hydrophilic–lypophilic balance (Oasis ® HLB) phase provides the best extraction yield of Levetiracetam, together with high precision. With the two other clean‐up procedures involving plasma deproteinization by addition of methanol or zinc sulphate, lower sensitivity and precision of the assays were obtained. However, they are cheaper and faster when compared with the solid‐phase extraction procedure. Copyright © 2003 John Wiley & Sons, Ltd.