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Isolation of volatiles from Nigella sativa seeds using microwave‐assisted extraction: effect of whole extracts on canine and murine CYP1A
Author(s) -
Liu Xue,
Park JongHyouk,
Abd ElAty A. M.,
Assayed M. E.,
Shimoda Minoru,
Shim JaeHan
Publication year - 2013
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.2887
Subject(s) - chemistry , thymoquinone , chromatography , nigella sativa , extraction (chemistry) , gas chromatography , in vivo , in vitro , biochemistry , traditional medicine , medicine , microbiology and biotechnology , biology , antioxidant
The volatile components of Nigella sativa seeds were isolated using microwave‐assisted extraction (MAE) and identified using gas chromatography. Further investigations were carried out to demonstrate the effects of whole extracts on canine (dog) and murine (rat) cytochrome P450 1A (CYP1A). The optimal extraction conditions of MAE were as follows: 25 mL of water, medium level of microwave oven power and 10 min of extraction time. A total of 32 compounds were identified under the conditions using GC‐FID and GC‐MS. Thymoquinone (38.23%), p ‐cymene (28.61%), 4‐isopropyl‐9‐methoxy‐1‐methyl‐1‐cyclohexene (5.74%), longifolene (5.33%), α ‐thujene (3.88) and carvacol (2.31%) were the main compounds emitted from N . sativa seeds. Various extracts including pure compounds, essential oil, nonpolar partition, relatively high‐polar/nonpolar partition, and polar partition extracts effectively inhibited the reaction of ethoxyresorufin O ‐de‐ethylation, which is specified for CYP1A activity both in dog and rat. This in vitro data should be heeded as a signal of possible in vivo interactions. The use of human liver preparations would considerably strengthen the practical impact of the data generated from this study. Copyright © 2013 John Wiley & Sons, Ltd.