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Simultaneous determination of cytosine arabinoside and its metabolite, uracil arabinoside, in human plasma using hydrophilic interaction liquid chromatography with UV detection
Author(s) -
Uchiyama Masanobu,
Takamatsu Yasushi,
Ogata Kentaro,
Matsumoto Taichi,
Jimi Shiro,
Tamura Kazuo,
Hara Shuuji
Publication year - 2013
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.2876
Subject(s) - chemistry , uracil , metabolite , cytosine , chromatography , human plasma , hydrophilic interaction chromatography , high performance liquid chromatography , biochemistry , dna
A practical high‐performance liquid chromatography using a Cosmosil HILIC column and UV detection was developed for determining the concentrations of cytosine arabinoside (Ara‐C) and uracil arabinoside (Ara‐U), which is a major metabolite of Ara‐C, in human plasma. This method was used to determine the plasma concentrations of Ara‐C and Ara‐U in a patient treated with high‐dose Ara‐C therapy for end‐stage renal failure. Copyright © 2013 John Wiley & Sons, Ltd.