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The use of linear ion trap for qualitative analysis of phytochemicals in Korean ginseng tea
Author(s) -
Stavrianidi Andrey,
Rodin Igor,
Braun Arkady,
Shpigun Oleg
Publication year - 2013
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.2857
Subject(s) - chemistry , quadrupole ion trap , chromatography , ion trap , ginseng , mass spectrometry , tandem mass spectrometry , electrospray ionization , qualitative analysis , selected reaction monitoring , quadrupole time of flight , analytical chemistry (journal) , medicine , qualitative research , social science , alternative medicine , pathology , sociology
A new approach to qualitative analysis of ginsenosides in challenging matrices was developed on the basis of high‐performance liquid chromatography/tandem mass spectrometry. Using the extracts from samples of ginseng tea, the approach was validated. Analysis of extracts was carried out using a reversed‐phase chromatography with SB‐C 18 sorbent. For compound identification, electrospray ionization and a quadrupole/linear ion trap mass‐spectrometer in different modes were used. A meticulous study of the fragmentation of ginsenosides in the linear ion trap and its application for analysis of these compounds was performed in this work. The accuracy of the identification was proven with standards of ginsenosides Rb1, Rg1, Re, Rf, Rd, Rb2, Rb3 and Rc. Copyright © 2012 John Wiley & Sons, Ltd.