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Indirect enantioseparation of proteinogenic amino acids using naproxen‐based chiral derivatizing reagent and HPLC
Author(s) -
Bhushan Ravi,
Nagar Hariom
Publication year - 2013
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.2855
Subject(s) - diastereomer , chemistry , chromatography , reagent , naproxen , detection limit , high performance liquid chromatography , benzotriazole , acetonitrile , chiral derivatizing agent , amino acid , chromatographic separation , organic chemistry , chiral column chromatography , medicine , biochemistry , alternative medicine , pathology
Diastereomers of 18 proteinogenic amino acids were synthesized under microwave irradiation and by vortexing using ( S )‐naproxen–benzotriazole as chiral derivatizing reagent. The diastereomers synthesized by two approaches were found to be identical in terms of their characterization and chromatographic data. A linear gradient of triethylammonium phosphate (pH 3.5)–acetonitrile (30–65%, within 35 min) was found to be successful using reversed‐phase high‐performance liquid chromatography for their separation. Detection was carried out at 231 nm and sharp peaks were obtained. The method was validated for accuracy, precision and limit of detection. Copyright © 2012 John Wiley & Sons, Ltd.