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A rapid and sensitive LC‐MS/MS method for the determination of osthole in rat plasma: application to pharmacokinetic study
Author(s) -
Yun Fei,
Kang An,
Shan Jinjun,
Zhao Xiaoli,
Bi Xiaolin,
Di Liuqing
Publication year - 2013
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.2850
Subject(s) - chemistry , chromatography , formic acid , electrospray ionization , pharmacokinetics , analyte , selected reaction monitoring , extraction (chemistry) , imperatorin , ethyl acetate , matrix (chemical analysis) , high performance liquid chromatography , mass spectrometry , tandem mass spectrometry , pharmacology , medicine
Osthole, a major component isolated from the fruit of Cnidium monnieri (L.) Cusson , has been widely used in traditional Chinese medicine. We developed and validated a rapid and sensitive LC‐MS/MS method for the quantification of osthole in rat plasma. Sample preparation involved simple liquid–liquid extraction by ethyl acetate after addition of imperatorin as internal standard (IS). The analyte was separated using a C 18 column with the mobile phase of methanol–0.1% formic acid (80:20, v/v) at a flow rate of 0.4 mL/min. The elutes were detected under positive electrospray ionization in multiple reaction monitoring mode. The method was sensitive with 0.5 ng/mL as the lower limit of detection. Good linearity was obtained over the range of 1.0–500.0 ng/mL. The intra and inter‐batch accuracy for osthole in rat plasma samples ranged from 99.5 to 108.1% and the variation was <8.9%. The stability, extraction efficiency and matrix effect were also acceptable. This method was successfully applied to the pharmacokinetic study of osthole in rat after intravenous and oral administration. Copyright © 2013 John Wiley & Sons, Ltd.