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Monolithic packed 96‐tips set for high‐throughput sample preparation: determination of cyclophosphamide and busulfan in whole blood samples by monolithic packed 96‐tips and LC‐MS
Author(s) -
Skoglund Christina,
Bassyouni Fatma,
AbdelRehim Mohamed
Publication year - 2013
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.2849
Subject(s) - busulfan , chromatography , chemistry , extraction (chemistry) , cyclophosphamide , calibration curve , sample preparation , analytical chemistry (journal) , calibration , detection limit , surgery , mathematics , chemotherapy , medicine , statistics
ABSTRACT A monolithic methacrylate packed 96‐tips device was used for the extraction of the busulfan and cyclophosphamide in whole blood samples. Using a packed 96‐tips set, a 96‐well plate could be handled in about 2 min. The key aspect of the monolithic phase is that monolithic material can offer both good extraction capacity and low‐back‐pressure properties. The validation of the methodology showed that the accuracy values of quality‐control samples were between 99 and 113% for busulfan, and between 103 and 110% for cyclophosphamide. The inter‐day precision ranged from 7.0 to 12% for busulfan and from 13 to 16% for cyclophosphamide. The standard calibration curves were obtained within the concentration range 5–2000 n m for busulfan and from 10 to 5000 n m for cyclophosphamide in blood samples. The coefficients of determination were ≥0.99. Copyright © 2012 John Wiley & Sons, Ltd.