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Low dose aspirin estimation: an application to a human pharmacokinetic study
Author(s) -
Vijaya Bharathi D.,
Hotha Kishore Kumar,
Kolagatla Pandu Ranga Reddy,
Venkateswarlu V.
Publication year - 2013
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.2832
Subject(s) - salicylic acid , aspirin , chemistry , pharmacokinetics , chromatography , detection limit , pharmacology , medicine , biochemistry
Low dose to very high dose aspirin is used to prevent heart attack. We have developed and validated a sensitive and robust method that could detect low levels of aspirin and salicylic acid in plasma and also a novel sample collection procedure to carry out sample preparation at room temperature. The total run time was 3.00 min; the developed method was validated in human plasma with a lower limit of quantitation of 0.99 ng/mL for aspirin and 2.01 ng/mL for salicylic acid. A linear response function was established for the range of concentrations 0.99–756.20 ng/mL ( r > 0.998) for aspirin and 2.01–2486.86 ng/mL for salicylic acid. The intra‐ and inter‐day precision values for aspirin and salicylic acid met the acceptance as per FDA guidelines. The developed assay method was applied to an oral pharmacokinetic study in humans. Copyright © 2012 John Wiley & Sons, Ltd.