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Determination of limonin in dog plasma by liquid chromatography–tandem mass spectrometry and its application to a pharmacokinetic study
Author(s) -
Liu Shijia,
Zhou Ling,
Zhang Jun,
Yu Boyang,
Li Changyin,
Liu Zixiu,
Ju Wenzheng
Publication year - 2013
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.2821
Subject(s) - chemistry , chromatography , limonin , selected reaction monitoring , analyte , ammonium acetate , mass spectrometry , liquid chromatography–mass spectrometry , tandem mass spectrometry , elution , extraction (chemistry) , pharmacokinetics , detection limit , high performance liquid chromatography , medicine
A highly sensitive liquid chromatography–tandem mass spectrometry method was developed and validated for the determination of limonin in beagle dog plasma using nimodipine as internal standard. The analyte and internal standard (IS) were extracted with ether followed by a rapid isocratic elution with 10 m m ammonium acetate buffer–methanol (26:74, v/v) on a C 18 column (150 × 2.1 mm i.d.) and subsequent analysis by mass spectrometry in the multiple reaction monitoring mode. The precursor to product ion transitions of m / z 469.4 → 229.3 and m / z 417.2 → 122.0 were used to measure the analyte and the IS. The assay was linear over the concentration range of 0.625–100 ng/mL for limonin in dog plasma. The lower limit of quantification was 0.312 ng/mL and the extraction recovery was >90.4% for limonin. The inter‐ and intra‐day precision of the method at three concentrations was less than 9.9%. The method was successfully applied to pharmacokinetic study of limonin in dogs. Copyright © 2012 John Wiley & Sons, Ltd.