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Hollow fiber‐based liquid membrane microextraction combined with high‐performance liquid chromatography for extraction and determination of trimetazidine in human plasma
Author(s) -
Lv Jiang,
Zhao Xu,
Ye Jing,
Liu Dan,
Chen Xiaohui,
Bi Kaishun
Publication year - 2013
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.2790
Subject(s) - chemistry , chromatography , trimetazidine , extraction (chemistry) , hollow fiber membrane , membrane , fiber , high performance liquid chromatography , plasma , biochemistry , organic chemistry , physics , quantum mechanics
A hollow fiber‐based liquid phase microextraction strategy combined with high‐performance liquid chromatography was evaluated for the quantitative determination of trimetazidine in human plasma. Trimetazidine was extracted from a 2.1 mL basified plasma sample (donor phase) into the organic solvent ( n ‐octanol) impregnated in the pores of a hollow fiber and then extracted into an acidic solution (acceptor phase) inside the lumen of the hollow fiber. The result showed that transport of drugs from alkaline sample solution into 0.5 m HCl occurred efficiently when 25 μL of 250 m m sodium 1‐octanesulfonate was added into the donor phase. Several parameters influencing the efficiency of the method, such as the nature of organic solvent used to impregnate the membrane, compositions of donor phase and acceptor phase, type and concentration of carrier, extraction time, stirring rate and salt concentration, were investigated and optimized. Under the optimal conditions, the calibration curves were obtained in the range of 5–200 ng/mL with reasonable linearity ( r > 0.9980). The method was successfully applied to determine the concentration of trimetazidine in human plasma. Copyright © 2012 John Wiley & Sons, Ltd.