Premium
Hollow fiber liquid‐phase microextraction and determination of nonsteroidal anti‐inflammatories by capillary electrophoresis and sulfonamides by HPLC in human urine
Author(s) -
Villar Navarro M.,
Ramos Payán M.,
FernándezTorres R.,
Bello López M. A.
Publication year - 2013
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.2783
Subject(s) - chemistry , chromatography , aceclofenac , capillary electrophoresis , naproxen , aqueous solution , high performance liquid chromatography , analyte , detection limit , extraction (chemistry) , urine , organic chemistry , medicine , biochemistry , alternative medicine , pathology
In this paper two applications of three‐phase HF‐LPME for the determination of pharmaceuticals in human urine are proposed: a capillary electrophoresis with a photodiode array detection method for the analysis of seven nonsteroidal anti‐inflammatory drugs (NSAIDs) and a high‐performance liquid chromatographic with photo diode array and fluorescence detection method for the determination of four sulfonamides and their corresponding N 4 ‐acetyl‐metabolites. Q3/2 Accurel® polypropylene hollow fibers were used for both procedures. Dihexyl ether was used as the supported liquid membrane for the determination of anti‐inflammatories and 1‐octanol for sulfonamides. An aqueous solution (pH 12) was used in both procedures as the acceptor phase and as the donor phase an aqueous solution (pH 2), and a 2 m Na 2 SO 4 aqueous solution (pH 4) was used for the determination of the anti‐inflammatories and sulfonamides. The detection limits obtained were between 0.25 (naproxen) and 0.86 ng/mL (aceclofenac) for the determination of anti‐inflammatories and 7 × 10 −4 (sulfamethoxazole) and 0.048 ng/mL (N 4 ‐acetyl‐sulfamethazine) for sulfonamides. The method was successfully applied to the determination of the analytes in human urine. Copyright © 2012 John Wiley & Sons, Ltd.