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A QuEChERS‐based extraction method for the residual analysis of pyraclofos and tebufenpyrad in perilla leaves using gas chromatography: application to dissipation pattern
Author(s) -
Al Mahmud M. N. U.,
Rahman Musfiqur,
Na TaeWoong,
Park JongHyouk,
Yang Angel,
Park Ki Hun,
Abd ElAty A. M.,
Nahar Nilufar,
Shim JaeHan
Publication year - 2013
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.2763
Subject(s) - quechers , chemistry , chromatography , gas chromatography , extraction (chemistry) , perilla , detection limit , pesticide residue , gas chromatography–mass spectrometry , derivatization , residual , electron capture detector , residue (chemistry) , mass spectrometry , pesticide , raw material , mathematics , biochemistry , organic chemistry , algorithm , agronomy , biology
ABSTRACT The objective of this work was to establish a simple extraction method for the residual analysis of pyraclofos and tebufenpyrad in Perilla leaves. A QuEChERS (quick, easy, cheap, effective, rugged and safe) method was used for extraction using ethyl acetate as an extraction solvent, and cleanup was carried out using dispersive solid‐phase extraction technique. The samples were analyzed using gas chromatography with nitrogen phosphorous detector and confirmed by gas chromatography–mass spectrometry. The linearity was excellent ( r 2 = 1.0) in matrix‐matched calibration for both pesticides. The recoveries at two fortification levels were 80.76–95.38% with relative standard deviation lower than 5%. The limits of detection and limits of quantification were 0.01 and 0.033 mg/kg for both pesticides, respectively. The results revealed that the dissipation pattern of pyraclofos and tebufenpyrad followed first‐order kinetics. The pyraclofos and tebufenpyrad residues declined to a level below the maximum residue limits within 14 day between the last application and harvesting. We suggest that pyraclofos and tebufenpyrad could be used efficiently on perilla leaves under the recommended dosage conditions. Copyright © 2012 John Wiley & Sons, Ltd.