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Preclinical pharmacokinetic analysis of armillarisin succinate ester in mouse plasma and tissues by LC–MS/MS
Author(s) -
Chen Qinhua,
Li Peng,
Zhang Jinfeng,
Zhu Jun
Publication year - 2013
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.2762
Subject(s) - chemistry , chromatography , ammonium acetate , electrospray ionization , analyte , pharmacokinetics , selected reaction monitoring , aqueous solution , methanol , mass spectrometry , liquid chromatography–mass spectrometry , tandem mass spectrometry , high performance liquid chromatography , pharmacology , medicine , organic chemistry
ABSTRACT A liquid chromatography–tandem mass spectrometry (LC–MS/MS) has been developed and validated to determine the concentration of armillarisin succinate ester in mouse plasma and tissues, used for preclinical evaluation. Bavachin was employed as the internal standard. Separation was performed on a 3.5 µm Zorbax SB‐C 18 column (30 × 2.1 mm), with a mobile phase consisting of methanol and aqueous 20 m m ammonium acetate. Both analyte and internal standard were determined using electrospray ionization and the MS data acquisition was via selected ion monitoring in negative scanning mode. Quantification was performed using the transitions m / z 333 → 233 and 323 → 221 for armillarisin succinate ester and internal standard, respectively. The method was validated with respect to linearity, accuracy, precision, recovery and stability. This assay has been successfully applied to a pharmacokinetic and tissue distribution study after intravenous injection of ASE in mouse in a dose of 10 mg/kg. Copyright © 2012 John Wiley & Sons, Ltd.